The company specializes in supplying Elisa kits, quality assurance, price concessions, and can provide free testing services, please call to consult! This manual is for reference only, subject to the company's current provision, please call to obtain it. This kit can only be used for scientific research, not for medical diagnosis. Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) Quantitative Detection Kit (ELISA) user's Guide ã€Naked mouse alpha fetoglobulin / alpha fetoprotein (AFP) ELISA kit kit name】 Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) Quantitative Detection Kit (ELISA) [Nude mouse alpha-fetoprotein / alpha-fetoprotein (AFP) ELISA kit kit uses] Quantitative detection of serum, plasma and related fluids. [Composition of Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit] 1 Enzyme coated plate 12 holes × 8 7 Developer A liquid 6mL 2 Standard 0.3mL × 6 tubes 8 Developer B liquid 6mL 3 20 times concentrated washing liquid 25mL 9 Stop solution 6mL 4 Sample diluent 6mL 10 Instructions 1 serving 5 Special diluent 6mL 11 Sealing film 2 sheets 6 Enzyme reagent 6mL 12 sealed bag 1 [Reagents and equipment not required by nude mouse alpha fetoglobulin / alpha fetoprotein (AFP) ELISA kit] 1. 37 ℃ thermostat 2. Standard specification microplate reader 3. Precision pipettes and disposable tips 4. Distilled water 5. Disposable test tubes 6. Absorbent paper [Nuclear Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit Detection Principle] This kit uses double-antibody two-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the standard and the test sample to the pre-coated plate coated with nude mice alpha-fetoprotein / alpha-fetoprotein (AFP) monoclonal antibody hyaluronidase. After incubating for a sufficient time, wash to remove unbound components. Add enzyme working solution again, and after incubation for a sufficient time, wash to remove unbound components. Substrates A and B were added in sequence, and the substrate (TMB) was converted into a blue product catalyzed by horseradish peroxidase (HRP), which turned yellow under the action of acid. The concentration of fetal globulin / alpha-fetoprotein (AFP) was positively correlated. The OD value was measured at a wavelength of 450 nm. Based on the OD value of the standard and the sample, the content of alpha-fetoglobulin / alpha-fetoprotein (AFP) in nude mice was calculated. [Precautions for Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit] 1. The experiment is carried out in strict accordance with the instructions, and the result of the experiment must be determined by the reading of the microplate reader. 2. If the enzyme-labeled coated board is not used up after opening, it should be put into a sealed bag and added with desiccant immediately. 3. It is recommended that all standards, samples and blank controls be tested in duplicate, and the average value is taken to reduce the experimental error. 4. Keep in mind that the sample has been diluted 5 times. The calculation result is multiplied by 5 to obtain the actual concentration of the sample. 5. The quantitative range of this kit is 6.25-200ng / L. If it exceeds this range, it is calculated from the extension of the standard curve. It is not used for accurate quantitative results. ), Multiplied by the total dilution factor is the final concentration of the sample. 6. If the color is too light, the substrate incubation time can be extended properly. 7. In order to avoid cross-contamination, the tips, samples and blank controls should be replaced with a new one for each addition; the common components such as enzyme working solution, sample diluent and substrate should be cantilevered, and they should not touch the microwells. ; Do not reuse the sealing film. 8. The kits are used within the warranty period, and different batches of reagents should not be mixed. 9. Substrate B is sensitive to light and avoid prolonged exposure to light. [Steps of Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit] 1. Preparation: Remove the reagent kit from the refrigerator and re-equilibrate at room temperature for 30 minutes. 2. Mixing solution: dilute the 20-fold concentrated washing solution with distilled water to the original one. 3. Add standard products and samples to be tested: take a sufficient number of enzyme-coated plates and fix them to the frame. Set up standard wells, sample wells to be tested and blank control wells, record the positions of each well in the standard wells. Add 50μL of standard product; first add 10μL of sample to be tested, and then add 40μL of sample diluent (that is, the sample is diluted 5 times); blank control well is not added. 4. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes. 5. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each hole with the washing solution, let stand for 1min, shake off the washing solution, pat dry on the absorbent paper, repeat the washing 4 times (you can also use the washing machine to press Instructions for washing the board). 6. Add enzyme-labeled working solution: add 50μL of enzyme-labeled working solution to each well, without adding blank control wells. 7. Incubation: Incubate in a 37 ° C water bath or thermostat for 30 minutes. 8. Wash the plate: discard the liquid, pat dry on the absorbent paper, fill each hole with washing liquid, let stand for 1min, shake off the washing liquid, pat dry on the absorbent paper, repeat the plate washing 4 times (you can also use the washing machine to press Instructions for washing the board). 9. Color development: add 50μL of developer A solution to each well, and then add 50μL of developer B solution, mix for 30s with a plate mixer (or gently shake for 30s by hand), and avoid color development at 37 ℃ for 15min . 10. Termination: Remove the enzyme labeling plate and add 50μL of stop solution to each well to stop the reaction (the color changes from blue to yellow) 11. Determination: Zero the blank holes, and within 15 minutes after termination, measure the absorbance (OD value) of each well with a wavelength of 450 nm. 12. Calculation: According to the concentration of the standard product and the corresponding OD value, calculate the linear regression equation of the standard curve, and then calculate the corresponding sample concentration on the regression equation according to the OD value of the sample. You can also use various application software to Calculation. The final concentration is the actual measured concentration times the dilution factor. [Sample Requirements for Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit] 1. The sample cannot contain sodium azide (NaN3) because sodium azide (NaN3) is an inhibitor of horseradish peroxidase (HRP). 2. The specimen should be extracted as soon as possible after collection. The extraction should be carried out according to relevant literature. If the test cannot be performed immediately, the specimen can be stored at -20 ℃, but repeated freezing and thawing should be avoided. 3. The sample should be fully centrifuged, without hemolysis and particles. [Summary of the operation procedures of Alpha Kit Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit in nude mice] Prepare reagents, samples and standards Add prepared samples and standards, and react at 37 ℃ for 30 minutes Wash the plate 4 times, add the enzyme reagent, and react at 37 ℃ for 30 minutes Wash the plate 4 times, add color developing solutions A and B, and develop at 37 ℃ for 15 minutes Add stop solution Read OD value within 15 minutes Calculation ã€Negative mouse alpha-fetoprotein / alpha-fetoprotein (AFP) ELISA kit detection range】 Please inquire [Specification of Alpha Kit Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit for Nude Mouse] 96 servings / box 48 servings / box ã€Storage of Nude Mouse Alpha Fetoglobulin / Alpha Fetoprotein (AFP) ELISA Kit】 Store at 2-8 ℃, protected from light and moisture. [Nude mouse alpha fetoglobulin / alpha fetoprotein (AFP) ELISA kit validity] 6 months
In
order to improve the clarity of the pattern and the photosensitive paste the
uniform distribution of the screen surface, the drying process should ensure
that no dust pollution around, and should be operating in the darkroom or yellow
light. Place the screen frame, printed side up, keep the screen level. Dust
free fan drying, but in order to speed up the drying speed drying box (Note:
the maximum temperature must not exceed 40 ° C).
Scratch coating layer is fully dry, wet surface will affect the light
sensitivity of the photosensitive paste must be done before the exposure. Not
completely dry, there will be underexposed
Drying operation guide:
3) often used in the industrial drying machine (maximum temperature 40 °
C), especially in the more humid environment. Must know, however, that the
expansion due to heating causes the screen frame chromatic inconvenience that
is difficult to achieve chromatic accurate. Therefore, in order to ensure that
the chromatic accurate, all wire mesh and network box must be dried in the same
environment. And they should be allowed to return to room temperature before
making an exposure. Plate-Drying Closet,Precision Plate-Drying Closet,Drying Closet For Pad Plate KC Printing Machine (Group) Limited , http://www.kcautopm.com
1) The third scratch coating method, each coated once must achieve sufficient
drying of a coating, otherwise, make moisture caught in the middle of the
photosensitive layer.
2) If possible, should be kept to a constant indoor temperature. If the indoor
humidity to extend the exposure time, especially in the summer, if not lower
humidity device, should pay special attention to this point. In fact, the cost
of installing lower humidity device is not too high
4) of the operating environment, should the exposure, dry place with the screen
frame making cleaning separately, because more humid in the production of wire
mesh and cleaning area