Lymphocyte separation liquid is a commonly used reagent for cell separation and purification based on the difference in cell density and the gravity acceleration generated by centrifugation. Commonly used lymphocyte separation fluids include Ficoll lymphocyte separation fluid and Percoll lymphocyte separation fluid. Both Ficoll and Percoll methods for separating mononuclear cells are density gradient centrifugation. 1. Ficoll-Hypaque density gradient centrifugation Ficoll is a polymer of sucrose, which is neutral, highly absorbent, and has an average molecular weight of 400,000. When the density is 1.2g / ml, it still does not exceed the normal physiological osmotic pressure and does not pass through the biofilm. Human peripheral blood mononuclear cells (PBMC) include lymphocytes and mononuclear cells. Their volume, shape, and specific gravity are different from other cells. Red blood cells and multinucleated white blood cells have a larger specific gravity of about 1.092, while lymphocytes and mononuclear cells The specific gravity of nuclear cells is about 1.075. When using a Ficoll-Hypaque mixed solution (called lymphocyte layered liquid) with a density of 1.077 ± 0.001g / L that is nearly isotonic for density gradient centrifugation, various blood components will be redistributed and aggregated according to the density gradient, red blood cells Due to the higher density of granulocytes, they sink to the bottom of the stratified fluid; the specific gravity of lymphocytes and monocytes is less than or equal to the specific gravity of the stratified fluid, so they are located at the interface of the stratified fluid so that PBMC can be obtained. In short: Ficoll can only separate lymphocytes with a density between 1.077 ± 0.001g / L 2. Percoll density gradient centrifugation: Percoll is composed of siliconized polyvinylpyrrolidone (PVP), which is a non-toxic and non-irritating new density gradient centrifugal separating agent. Polyvinylpyrrolidone-coated silica gel suspension has the advantages of easy isotonicity, low viscosity, non-toxicity, and does not cause cell aggregation. It has been widely cited. Because Percoll has different particle sizes in the medium, it naturally forms a density gradient during centrifugation, thereby separating cells of different densities. Mix 1 part of 10 × PBS with 9 parts of percoll stock solution to make an isotonic Percoll suspension. This suspension is considered to be a 100% Percoll suspension with a density of 1.1294g / ml. Tissue culture solution dilutes this 100% Percoll to obtain a suitable density for cell separation, because the dilution is linearly related to the density, the commonly used density is as follows: 70% Percoll 1.090g / ml 60% Percoll 1.077g / ml 50% Percoll 1.067g / ml 40% Percoll 1.056g / ml 30% Percoll 1.043g / ml 20% Percoll 1.031g / ml Through the superposition of several high to low dilutions, a gradient corresponding to the specific gravity of the cells of the mixed cells is formed, so that cells of different densities can be distinguished. In short: PERCOLL can separate a variety of cells in whole blood. Candy Chocolate Chewing Gum Display Rack Candy Chocolate Chewing Gum Display Rack,Countertop Snacks Display Rack,Metal Rack For Food,Display Rack For Chocolate Dongguan Display Leader Co., Ltd , https://www.displayonestop.com